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Elife. 2019 Oct 18;8. pii: e49291. doi: 10.7554/eLife.49291.

Monosynaptic tracing maps brain-wide afferent oligodendrocyte precursor cell connectivity.

Author information

1
Department of Neurology, Stanford University, Stanford, United States.
2
Medical Scientist Training Program, Stanford University, Stanford, United States.
3
Neurosciences Graduate Program, Stanford University, Stanford, United States.
4
Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, United States.
5
Department of Pathology, Stanford University, Stanford, United States.
6
Department of Pediatrics, Stanford University, Stanford, United States.
#
Contributed equally

Abstract

Neurons form bona fide synapses with oligodendrocyte precursor cells (OPCs), but the circuit context of these neuron to OPC synapses remains incompletely understood. Using monosynaptically-restricted rabies virus tracing of OPC afferents, we identified extensive afferent synaptic inputs to OPCs residing in secondary motor cortex, corpus callosum, and primary somatosensory cortex of adult mice. These inputs primarily arise from functionally-interconnecting cortical areas and thalamic nuclei, illustrating that OPCs have strikingly comprehensive synaptic access to brain-wide projection networks. Quantification of these inputs revealed excitatory and inhibitory components that are consistent in number across brain regions and stable in barrel cortex despite whisker trimming-induced sensory deprivation.

KEYWORDS:

axon-glial synapse; monosynaptic viral tracing; mouse; myelin; neuron-glia interactions; neuroscience; oligodendrocyte precursor cell

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