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Med Sci (Paris). 2019 Oct;35(10):761-770. doi: 10.1051/medsci/2019154. Epub 2019 Oct 18.

[Whole genome transplantation: bringing natural or synthetic bacterial genomes back to life].

[Article in French; Abstract available in French from the publisher]

Author information

1
Institute of Veterinary Bacteriology, University of Bern, PO Box, CH-3001 Bern, Suisse.
2
UMR 1332 Biologie du fruit et pathologie, INRA Bordeaux-Aquitaine, 71 avenue E. Bourlaux, 33882 Villenave d'Ornon, France.
3
Département de biologie, Université de Sherbrooke, 2500 boulevard de l'université, Sherbrooke, Québec, Canada.

Abstract

in English, French

The development of synthetic genomics (SG) allowed the emergence of several groundbreaking techniques including the synthesis, assembly and engineering of whole bacterial genomes. The successful implantation of those methods, which culminated in the creation of JCVI-syn3.0 the first nearly minimal bacterium with a synthetic genome, mainly results from the use of the yeast Saccharomyces cerevisiae as a transient host for bacterial genome replication and modification. Another method played a key role in the resounding success of this project: bacterial genome transplantation (GT). GT consists in the transfer of bacterial genomes cloned in yeast, back into a cellular environment suitable for the expression of their genetic content. While successful using many mycoplasma species, a complete understanding of the factors governing GT will most certainly help unleash the power of the entire SG pipeline to other genetically intractable bacteria.

PMID:
31625898
DOI:
10.1051/medsci/2019154

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