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Sci Adv. 2019 Oct 2;5(10):eaaw6264. doi: 10.1126/sciadv.aaw6264. eCollection 2019 Oct.

Gold-DNA nanosunflowers for efficient gene silencing with controllable transformation.

Author information

1
CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11 First North Road, Zhongguancun, Beijing 100190, P. R. China.
2
DWI-Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52056 Aachen, Germany.
3
University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
4
Department of Chemistry, University of Florida, Gainesville, FL 32611, USA.
5
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
6
Shijiazhuang Zangnuo Bio Incorporated Corporation, No. 518 Cangsheng Road, Hi-tech District, Shijiazhuang, Hebei Province 050000, P. R. China.

Abstract

The development of an efficient delivery system for enhanced and controlled gene interference-based therapeutics is still facing great challenges. Fortunately, the flourishing field of nanotechnology provides more effective strategies for nucleic acid delivery. Here, the triplex-forming oligonucleotide sequence and its complementary strand were used to mediate self-assembly of ultrasmall gold nanoparticles. The obtained sunflower-like nanostructures exhibited strong near-infrared (NIR) absorption and photothermal conversion ability. Upon NIR irradiation, the large-sized nanostructure could disassemble and generate ultrasmall nanoparticles modified with c-myc oncogene silencing sequence, which could directly target the cell nucleus. Moreover, the controlled gene silencing effect could be realized by synergistically controlling the preincubation time with the self-assembled nanostructure (in vitro and in vivo) and NIR irradiation time point. This study provides a new approach for constructing more efficient and tailorable nanocarriers for gene interference applications.

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