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Curr Opin Biotechnol. 2019 Oct 10;64:24-31. doi: 10.1016/j.copbio.2019.09.002. [Epub ahead of print]

Systematic mapping of protein-metabolite interactions with mass spectrometry-based techniques.

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iHuman Institute, ShanghaiTech University, 201210, Shanghai, China.
iHuman Institute, ShanghaiTech University, 201210, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China. Electronic address:


The recent rapid advance of systematic mapping of protein-metabolite interactions (PMIs) in both prokaryotic and eukaryotic cells has been catalyzed by development of innovative and effective proteomics or metabolomics strategies all based on large-scale mass spectrometry (MS) analysis of biomolecules. Both metabolite-centric and protein-centric approaches have been established to profile PMIs in the native cellular matrix treated by specific metabolites or proteins. Here we will review the development and application of versatile MS-based proteomics and metabolomics techniques for global PMI mapping in different species, which lead to the discovery of numerous uncharacterized PMIs that may reveal new interaction-derived functionality. We further discuss the strengths and limitations of different PMI mapping approaches as well as the key elements in MS quantification and data mining for reliable PMI identification.

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