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J Cancer Res Ther. 2019 Jul-Sep;15(5):1162-1166. doi: 10.4103/jcrt.JCRT_803_16.

Aberrant signal transduction in Indian triple-negative breast cancer patients.

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Department of Biochemistry, RCASC, Bengaluru, Karnataka, India.
Department of Pathology, RMCH, Bengaluru, Karnataka, India.


Aim of Study:

The aim of this study is to correlate the prominin-1 or CD133 association with functional pathway markers of cancer stemness in Indian triple-negative breast cancer (TNBC) patient samples.

Materials and Methods:

TNBC samples were confirmed for the absence of hormone receptors (estrogen receptor-ER/progesterone receptor) and human epidermal growth factor receptor-2 or proto-oncogene neu or erbB2 or CD340 by immunohistochemical analysis. Formalin-fixed paraffin-embedded samples of patients were used to collect the total RNA. Then, one-step reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the cancer stemness-related transcript levels in the different samples. The RT-PCR products were analyzed semi-quantitatively on agarose gels. The band intensities of respective samples for different transcripts were analyzed by densitometry.


TNBC-confirmed samples had shown increased levels of CD133 transcript than control tissues. Further, elevated CD133 transcripts are correlated with higher transcript levels of NOTCH1/FZD7/transforming growth factor-beta receptor Type III R/patched-1 pathway mediators.


This work has clearly indicated that there is a correlation between CD133 and functional pathways that control cancer stem cells in TNBC. These observations may indicate the possible association between cancer stemness and TNBC malignancy.


Cancer stem cells; FZD7; NOTCH1; patched-1; signal transduction; transforming growth factor-beta receptor Type III R; triple-negative breast cancer

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