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J Cancer Res Ther. 2019 Jul-Sep;15(5):1098-1104. doi: 10.4103/jcrt.JCRT_381_17.

Antiproliferative and apoptotic effects of the natural alkaline water (Zamzam) in breast cancer cell line MCF-7.

Author information

1
Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
2
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University; KFMRC, Immunology Unit, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Background:

Zamzam water (ZW) is a natural alkaline water that contains several minerals that may represent a powerful tool for cancer therapy.

Objectives:

In this research, in vitro antiproliferative and apoptotic effects of ZW were investigated in the human breast cancer cell line MCF-7.

Materials and Methods:

This study was conducted between January 2015 and February 2016. The effects of ZW on the morphology and the cell viability of human breast cancer cell line MCF-7 were determined. The cell death type and cell cycle changes were investigated using flow cytometry. Finally, reactive oxygen species (ROS) were also measured by fluorometric technique.

Results:

MCF-7 cells treated with either ZW with adjusted pH at 7.2 or unadjusted pH at 8 showed reduced cell viability of cancerous cells. The cell death occurred through the apoptosis pathway under both treatment conditions. The treated MCF-7 cells were arrested in the G2/M phase and decreased in the G1 phase. Only the unadjusted pH ZW sample demonstrated an increase in the production of both cytoplasmic and mitochondrial ROS in MCF-7 cells.

Conclusion:

All the results in the present study indicated, for the first time, that ZW might have anticancer and apoptotic effects on breast cancer cell line.

KEYWORDS:

Antiproliferation; MCF-7; Zamzam water; apoptosis; cell cycle; reactive oxygen species

PMID:
31603117
DOI:
10.4103/jcrt.JCRT_381_17
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