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J Cancer Res Ther. 2019 Jul-Sep;15(5):1092-1097. doi: 10.4103/jcrt.JCRT_1227_16.

Circulating miR-21 and miR-155 as potential noninvasive biomarkers in Iranian Azeri patients with breast carcinoma.

Author information

1
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
2
Department of Thorax Surgery, Noor Nejat Hospital, Tabriz, Iran.

Abstract

Background:

Breast cancer (BC) is the most common cause of cancer-related mortality among women. Despite recent advances in diagnosis and prognosis of breast carcinomas, noninvasive biomarkers have been poorly identified. We evaluated the biomarker potential of miR-21 and miR-155 in tissue and plasma specimens of Iranian Azeri patients.

Materials and Methods:

Tumor specimens, paired nontumoral adjacent tissues, and matched plasma samples were collected from a number of thirty Iranian Azeri women with breast carcinoma. Plasma of healthy women was used as the control. The relative expression of miR-21 and miR-155 was measured by real-time polymerase chain reaction.

Results:

Our data revealed that the expression levels of miR-21 and miR-155 in tumor tissues are significantly higher than paired nontumoral adjacent specimens (P < 0.05). Furthermore, receiver operating characteristic (ROC) curve analysis of samples showed the area under the ROC curve of 0.81 for miR-21 and area of 0.83 for miR-155. In addition, statistical analysis showed that miR-21 and miR-155 RNAs are significantly detected in the plasma of BC patients compared to healthy specimens (P < 0.05). Circulating miRNAs yielded area under the ROC curve of 0.99 for miR-21 and 0.92 for miR-155.

Conclusion:

Our data showed that miR-21 and miR-155 oncomiRs can be considered as noninvasive biomarkers for monitoring breast carcinomas. However, further investigations are needed to confirm the use of these noncoding RNAs in pathology.

KEYWORDS:

Biomarkers; breast; early detection of cancer; microRNAs; prognosis

PMID:
31603116
DOI:
10.4103/jcrt.JCRT_1227_16
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