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Wei Sheng Yan Jiu. 2019 Sep;48(5):803-806.

[Effects of equol on proliferation of colorectal cancer HCT-15 cell].

[Article in Chinese]

Author information

1
Department of Social Medicine and Health Education, School of Public Health, Peking University, Beijing 100191, China.

Abstract

OBJECTIVE:

To investigate the effect of racemic equol and equol enantiomers on the proliferation of colorectal cancer HCT-15 cell and the potential mechanism.

METHODS:

3-( 4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide( MTT) assay was used to detect the effect of different concentrations of racemic equol and equol enantiomers( 0, 0. 5, 1, 5 and 10 μmol/L) on the proliferation of colorectal cancer HCT-15 cell. Western blot was used to detect the expression of estrogen receptor( ER)and nuclear factor 2 related factor 2( Nrf2).

RESULTS:

Racemic equol and( R) equol inhibited the proliferation of HCT-15 cell in a dose-dependent manner, whereas( S) equol had no effect on the proliferation of HCT-15 cell. Racemic equol increased the expression of ERβ and Nrf2, while( R) equol increased the expression of Nrf2.

CONCLUSION:

Racemic equol can inhibit the proliferation of HCT-15 cell through estrogenic and antioxidative activity. R equol can inhibit the proliferation of HCT-15 cell through antioxidative activity, while( S) equol has no effect on the proliferation of HCT-15 cell.

KEYWORDS:

HCT-15 cell; colorectal cancer; equol; equol enantiomers; estrogen receptor

PMID:
31601322
[Indexed for MEDLINE]

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