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Diabetes. 2019 Oct 9. pii: db190554. doi: 10.2337/db19-0554. [Epub ahead of print]

Temporal Analysis of Amylase Expression in Control, Autoantibody Positive, and Type 1 Diabetes Pancreatic Tissues.

Author information

1
Department of Pathology, Immunology and Laboratory Medicine, Diabetes Institute University of Florida, Gainesville, FL, USA.
2
Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL, USA.
3
Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL, USA.
4
Department of Pathology, Immunology and Laboratory Medicine, Diabetes Institute University of Florida, Gainesville, FL, USA atkinson@ufl.edu.

Abstract

Within the human pancreas, exocrine and endocrine cells control secretion of digestive enzymes and production of hormones to maintain metabolic homeostasis, respectively. While the vast majority of type 1 diabetes research efforts have focused on endocrine function and autoimmunity, recent studies identified a series of unique features (e.g., reduced weight and volume, increased density of leukocytes) within the exocrine pancreas in this disease, but the mechanisms underlying these aberrancies are unknown. Therefore, we histologically assessed amylase, insulin, glucagon, lipase and/or trypsinogen in 78 organ donor pancreata, from birth through adulthood in controls and those at various stages of type 1 diabetes. While amylase-positive acinar cells were detectable in pancreata from all study groups, tissues from individuals over two years of age contained clusters of acinar cells devoid of amylase. A majority of these amylase-negative (AMY-) cell clusters localized proximal to islets (i.e., peri-islet). Additionally, most AMY- clusters were positive for the exocrine enzymes lipase and trypsinogen. Interestingly, type 1 diabetes pancreata displayed significant reductions in the frequency of these AMY- cell clusters. These results support a contribution of the islet-acinar axis in pancreatic development and underscore a potential role for the exocrine pancreas in the pathogenesis of type 1 diabetes.

PMID:
31597639
DOI:
10.2337/db19-0554

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