Format

Send to

Choose Destination
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):882-894. doi: 10.1107/S2059798319011860. Epub 2019 Oct 1.

Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion.

Author information

1
National Center for Biotechnology (CSIC), 28049 Cantoblanco, Madrid, Spain.
2
MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, England.
3
Universidad CEU San Pablo, Madrid, Spain.
4
Universidad Autónoma de Madrid, Madrid, Spain.

Abstract

Electron microscopy of macromolecular structures is an approach that is in increasing demand in the field of structural biology. The automation of image acquisition has greatly increased the potential throughput of electron microscopy. Here, the focus is on the possibilities in Scipion to implement flexible and robust image-processing workflows that allow the electron-microscope operator and the user to monitor the quality of image acquisition, assessing very simple acquisition measures or obtaining a first estimate of the initial volume, or the data resolution and heterogeneity, without any need for programming skills. These workflows can implement intelligent automatic decisions and they can warn the user of possible acquisition failures. These concepts are illustrated by analysis of the well known 2.2 Å resolution β-galactosidase data set.

KEYWORDS:

Scipion; electron microscopy; electron-microscopy facilities; image processing; single-particle analysis; stream processing

PMID:
31588920
DOI:
10.1107/S2059798319011860

Supplemental Content

Full text links

Icon for International Union of Crystallography
Loading ...
Support Center