Chemical modification of pure titanium surfaces to enhance the cytocompatibility and differentiation of human mesenchymal stem cells

Dent Mater J. 2019 Dec 1;38(6):1026-1035. doi: 10.4012/dmj.2018-257. Epub 2019 Oct 2.

Abstract

The aim of this study was to improve the cytocompatibility and differentiation of human bone marrow-derived mesenchymal stem cells on the surface of titanium implants by immobilizing biofunctional molecules on their surface. Gly-Arg-Gly-Asp-Ser (GRGDS) peptides, human plasma fibronectin (pFN), or type I collagen from calf skin (Col) was covalently immobilized on the titanium surfaces. Twice as many cells attached to the Col- and pFN-immobilized titanium surfaces than attached to the as-polished surface control. The ALP activity of the cells, as well as the mineralized nodule formation, was significantly higher on the Col- and pFN-immobilized titanium surfaces than on the as-polished surfaces. These results indicate that the immobilization of biofunctional molecules such as Col and pFN on titanium surfaces enhances the attachment, spreading, proliferation, and differentiation of human bone marrow-derived mesenchymal stem cells, which may lead to a more rapid bone-titanium integration.

Keywords: Biofunctional molecules; Chemical modification; Human bone marrow-derived mesenchymal stem cells; Implant; Titanium.

MeSH terms

  • Cell Adhesion
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Collagen Type I
  • Humans
  • Mesenchymal Stem Cells*
  • Osteoblasts
  • Osteogenesis
  • Surface Properties
  • Titanium

Substances

  • Collagen Type I
  • Titanium