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iScience. 2019 Sep 18;20:184-194. doi: 10.1016/j.isci.2019.09.022. [Epub ahead of print]

Longitudinal In Vivo Assessment of Host-Microbe Interactions in a Murine Model of Pulmonary Aspergillosis.

Author information

1
Biomedical MRI/Molecular Small Animal Imaging Center (MoSAIC), KU Leuven, Leuven, Belgium.
2
Laboratory of Hepatology, CHROMETA Department, KU Leuven, Leuven, Belgium.
3
Laboratory of Genetics of Autoimmunity (VIB-KU Leuven Center for Brain & Disease Research), Leuven, Belgium.
4
Biomedical MRI/Molecular Small Animal Imaging Center (MoSAIC), KU Leuven, Leuven, Belgium; Philips Research China, Shanghai, China.
5
Ghent Research Group on Nanomedicines, Ghent University, Belgium.
6
Clinical Bacteriology and Mycology, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.
7
Clinical and Experimental Endocrinology, KU Leuven, Leuven, Belgium.
8
Biomedical MRI/Molecular Small Animal Imaging Center (MoSAIC), KU Leuven, Leuven, Belgium. Electronic address: uwe.himmelreich@kuleuven.be.

Abstract

The fungus Aspergillus fumigatus is ubiquitous in nature and the most common cause of invasive pulmonary aspergillosis (IPA) in patients with a compromised immune system. The development of IPA in patients under immunosuppressive treatment or in patients with primary immunodeficiency demonstrates the importance of the host immune response in controlling aspergillosis. However, study of the host-microbe interaction has been hampered by the lack of tools for their non-invasive assessment. We developed a methodology to study the response of the host's immune system against IPA longitudinally in vivo by using fluorine-19 magnetic resonance imaging (19F MRI). We showed the advantage of a perfluorocarbon-based contrast agent for the in vivo labeling of macrophages and dendritic cells, permitting quantification of pulmonary inflammation in different murine IPA models. Our findings reveal the potential of 19F MRI for the assessment of rapid kinetics of innate immune response against IPA and the permissive niche generated through immunosuppression.

KEYWORDS:

Infection Control in Health Technology; Medical Imaging; Mycology

PMID:
31581067
DOI:
10.1016/j.isci.2019.09.022
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