Biogenic silver nanoparticles as a more efficient contrivance for wound healing acceleration than common antiseptic medicine

FEMS Microbiol Lett. 2019 Aug 1;366(16):fnz201. doi: 10.1093/femsle/fnz201.

Abstract

A simple and facile way of using biogenic silver nanoparticles (BSNP) (10-20 nm) was developed for wound healing acceleration and suppression of wound infections. The BSNP were formulated in an ointment base, and the study to accelerate the wound healing process was conducted in a rat. The pH of the BSNP ointment, pH 6.8 ± 0.5, lies in normal pH range of the human skin, with good spreadability and diffusibility. The wound closure rate, as a percentage, was highest at day 3 for a BSNP ointment-treated wound at 22.77 ± 1.60%, while in an untreated control the rate was 10.99 ± 1.74%, for Betadine 14.73 ± 2.36% and for Soframycin 18.55 ± 1.37%, compared with day 0. A similar pattern of wound closure rate was found at days 7 and 11. The antibacterial activity of BSNP was evaluated against wound-infection-causing bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli by the agar diffusion method. The total bacterial counts in the wound area were enumerated by the colony forming unit method. The lowest number of bacterial counts was found in the BSNP-treated wound compared with the other groups. BSNP treatment at 7.5% concentration enhanced migration of fibroblasts in a scratch assay. These findings reveal BSNP as an efficient contrivance for wound healing acceleration and as an eco-friendly alternative therapeutic antimicrobial agent.

Keywords: antibacterial activity; biogenic silver nanoparticles; common antiseptic medicine; re-epithelialization; wound healing acceleration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Infective Agents / administration & dosage*
  • Colony Count, Microbial
  • Disease Models, Animal
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / prevention & control
  • Metal Nanoparticles / administration & dosage*
  • Nanomedicine / methods*
  • Ointments / administration & dosage
  • Pseudomonas Infections / prevention & control
  • Pseudomonas aeruginosa / isolation & purification
  • Rats
  • Silver / administration & dosage*
  • Staphylococcal Infections / prevention & control
  • Staphylococcus aureus / isolation & purification
  • Treatment Outcome
  • Wound Healing / drug effects*
  • Wound Infection / drug therapy
  • Wound Infection / microbiology
  • Wound Infection / prevention & control*
  • Wounds and Injuries / drug therapy*
  • Wounds and Injuries / microbiology

Substances

  • Anti-Infective Agents
  • Ointments
  • Silver