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Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 201910840. doi: 10.1073/pnas.1910840116. [Epub ahead of print]

X chromosome and autosomal recombination are differentially sensitive to disruptions in SC maintenance.

Author information

1
Stowers Institute for Medical Research, Kansas City, MO 64110.
2
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403.
3
Department of Biology, University of Oregon, Eugene, OR 97403.
4
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160.
5
University of Missouri-Kansas City, Kansas City, MO 64110.
6
Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan 192-0397.
7
Stowers Institute for Medical Research, Kansas City, MO 64110; rsh@stowers.org.

Abstract

The synaptonemal complex (SC) is a conserved meiotic structure that regulates the repair of double-strand breaks (DSBs) into crossovers or gene conversions. The removal of any central-region SC component, such as the Drosophila melanogaster transverse filament protein C(3)G, causes a complete loss of SC structure and crossovers. To better understand the role of the SC in meiosis, we used CRISPR/Cas9 to construct 3 in-frame deletions within the predicted coiled-coil region of the C(3)G protein. Since these 3 deletion mutations disrupt SC maintenance at different times during pachytene and exhibit distinct defects in key meiotic processes, they allow us to define the stages of pachytene when the SC is necessary for homolog pairing and recombination during pachytene. Our studies demonstrate that the X chromosome and the autosomes display substantially different defects in pairing and recombination when SC structure is disrupted, suggesting that the X chromosome is potentially regulated differently from the autosomes.

KEYWORDS:

Drosophila; homologous recombination; meiosis; synaptonemal complex

PMID:
31570610
DOI:
10.1073/pnas.1910840116

Conflict of interest statement

The authors declare no competing interest.

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