Format

Send to

Choose Destination
Int J Mol Sci. 2019 Sep 27;20(19). pii: E4815. doi: 10.3390/ijms20194815.

Decorin Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induces Macrophage Polarization via CD44 to Repair Hyperoxic Lung Injury.

Author information

1
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. kjh0127@medi-post.co.kr.
2
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. eldjfls3@medi-post.co.kr.
3
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. byk819@medi-post.co.kr.
4
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. haha38@medi-post.co.kr.
5
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. sjchoi@medi-post.co.kr.
6
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. wioh@medi-post.co.kr.
7
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. ssoso23@medi-post.co.kr.
8
Biomedical Research Institute, MEDIPOST Co., Ltd., Seongnam 13494, Korea. genny77@medi-post.co.kr.

Abstract

Bronchopulmonary dysplasia (BPD), caused by hyperoxia in newborns and infants, results in lung damage and abnormal pulmonary function. However, the current treatments for BPD are steroidal and pharmacological therapies, which cause neurodevelopmental impairment. Treatment with umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) is an efficient alternative approach. To prevent pulmonary inflammation in BPD, this study investigated the hypothesis that a key regulator was secreted by MSCs to polarize inflammatory macrophages into anti-inflammatory macrophages at inflammation sites. Lipopolysaccharide-induced macrophages co-cultured with MSCs secreted low levels of the inflammatory cytokines, IL-8 and IL-6, but high levels of the anti-inflammatory cytokine, IL-10. Silencing decorin in MSCs suppressed the expression of CD44, which mediates anti-inflammatory activity in macrophages. The effects of MSCs were examined in a rat model of hyperoxic lung damage. Macrophage polarization differed depending on the levels of decorin secreted by MSCs. Moreover, intratracheal injection of decorin-silenced MSCs or MSCs secreting low levels of decorin confirmed impaired alveolarization of damaged lung tissues by down-regulation of decorin. In tissues, a decrease in the anti-inflammatory macrophage marker, CD163, was observed via CD44. Thus, we identified decorin as a key paracrine factor, inducing macrophage polarization via CD44, a master immunoregulator in mesenchymal stem cells.

KEYWORDS:

CD44; bronchopulmonary dysplasia; decorin; macrophage polarization; mesenchymal stem cell

PMID:
31569732
PMCID:
PMC6801980
DOI:
10.3390/ijms20194815
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center