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Biomater Sci. 2019 Nov 1;7(11):4708-4719. doi: 10.1039/c9bm00539k. Epub 2019 Sep 30.

Yeast glucan particles enable intracellular protein delivery in Drosophila without compromising the immune system.

Author information

1
University of South Bohemia, Faculty of Sciences, Department of Molecular Biology and Genetics, Branišovska 1160/31, 37005 České Budějovice, Czech Republic and University of Chemistry and Technology Prague, Department of Chemical Engineering, Technická 3, 166 28 Prague, Czech Republic. Frantisek.Stepanek@vscht.cz.
2
University of Chemistry and Technology Prague, Department of Chemical Engineering, Technická 3, 166 28 Prague, Czech Republic. Frantisek.Stepanek@vscht.cz.
3
University of South Bohemia, Faculty of Sciences, Department of Molecular Biology and Genetics, Branišovska 1160/31, 37005 České Budějovice, Czech Republic.
4
Biology Centre CAS, Institute of Entomology, Branišovská 1160/31, 37005 České Budějovice, Czech Republic.

Abstract

Glucan particles derived from yeast have been recently proposed as potential drug delivery carriers. Here, we demonstrate the potential of glucan particles for protein delivery in vivo, using the insect Drosophila melanogaster as a model organism. By employing genetic tools, we demonstrate the capacity of yeast glucan particles to spread efficiently through the Drosophila body, to enter macrophages and to deliver an active transcription factor protein successfully. Moreover, the glucan particles were nontoxic and induced only minimal immune response. The injection of glucan particles did not impair the ability of Drosophila to fight and survive infection by pathogenic bacteria. From this study, Drosophila emerges as an excellent model to test and develop drug delivery systems based on glucan particles, specifically aimed to regulate macrophages.

PMID:
31565713
DOI:
10.1039/c9bm00539k

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