Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant

Xiawei Jiang; Xinjie Cui; Wenhong Liu; Hao Xu; Beiwen Zheng

doi:10.2147/IDR.S213525

Genetic characterization of a novel sequence type of multidrug-resistant Citrobacter freundii strain recovered from wastewater treatment plant

Infect Drug Resist. 2019 Sep 6:12:2775-2779. doi: 10.2147/IDR.S213525. eCollection 2019.

Authors

Xiawei Jiang¹, Xinjie Cui¹, Wenhong Liu¹, Hao Xu², Beiwen Zheng²

Affiliations

¹ College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China.
² Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

Abstract

A multidrug-resistant Citrobacter freundii strain R17 was isolated from a wastewater treatment plant in China. Whole-genome sequencing of strain R17 revealed a new sequence type (ST412) chromosome (length 5,124,258 bp) and an Inc FII (Yp) group plasmid pCFR17_1 (length 206,820 bp). A total of 13 antibiotic-resistance genes (ARGs) that confer resistance to eight different antibiotic groups were encoded by strain R17 and 12 of them were carried by plasmid pCFR17_1. These data and analysis suggest that the environment-derived C. freundii strains may serve as potential sources of ARGs and highlight the need of further surveillance of this bacteria in the future.

Keywords: Citrobacter freundii; antibiotic-resistance genes; insertion elements; multidrug resistant; wastewater treatment plant; whole-genome sequencing.