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Mol Cells. 2019 Sep 30;42(9):637-645. doi: 10.14348/molcells.2019.0070.

Pi5 and Pii Paired NLRs Are Functionally Exchangeable and Confer Similar Disease Resistance Specificity.

Author information

1
Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
2
These authors contributed equally to this work.
3
Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Korea.
4
Present address: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.
5
Department of Plant Medicine, Sunchon National University, Suncheon 57922, Korea.
6
Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Korea.
7
Department of Plant Bioscience, Pusan National University, Miryang 46241, Korea.
8
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 37673, Korea.

Abstract

Effector-triggered immunity (ETI) is an effective layer of plant defense initiated upon recognition of avirulence (Avr) effectors from pathogens by cognate plant disease resistance (R) proteins. In rice, a large number of R genes have been characterized from various cultivars and have greatly contributed to breeding programs to improve resistance against the rice blast pathogen Magnaporthe oryzae. The extreme diversity of R gene repertoires is thought to be a result of co-evolutionary history between rice and its pathogens including M. oryzae. Here we show that Pii is an allele of Pi5 by DNA sequence characterization and complementation analysis. Pii-1 and Pii-2 cDNAs were cloned by reverse transcription polymerase chain reaction from the Pii -carrying cultivar Fujisaka5 . The complementation test in susceptible rice cultivar Dongjin demonstrated that the rice blast resistance mediated by Pii , similar to Pi5 , requires the presence of two nucleotide-binding leucine-rich repeat genes, Pii-1 and Pii-2 . Consistent with our hypothesis that Pi5 and Pii are functionally indistinguishable, the replacement of Pii-1 by Pi5-1 and Pii-2 by Pi5-2 , respectively, does not change the level of disease resistance to M. oryzae carrying AVR-Pii. Surprisingly, Exo70F3, required for Pii-mediated resistance, is dispensable for Pi5-mediated resistance. Based on our results, despite similarities observed between Pi5 and Pii, we hypothesize that Pi5 and Pii pairs require partially distinct mechanisms to function.

KEYWORDS:

Magnaporthe oryzae; Pi5; Pii; allelism; resistance; rice

PMID:
31564075
DOI:
10.14348/molcells.2019.0070
Free PMC Article

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