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Mutat Res. 2019 Sep;845:402993. doi: 10.1016/j.mrgentox.2018.12.001. Epub 2018 Dec 21.

Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro.

Author information

1
Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: dijana.topalovic@pharmacy.bg.ac.rs.
2
Biomedical Research, R&D Institute, Galenika a.d., Pasterova 2, 11000 Belgrade, Serbia. Electronic address: ddekan@sezampro.rs.
3
Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: biljana.potparevic@pharmacy.bg.ac.rs.
4
Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: andrea@pharmacy.bg.ac.rs.
5
Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia. Electronic address: sborozan@vet.bg.ac.rs.
6
The Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research "Vinča", University of Belgrade, Mike Petrovića Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: vladanbajic@yahoo.com.
7
Department of Botany, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: danilo.stojanovic@pharmacy.bg.ac.rs.
8
Department of Odontostomatologic and Specialized Clinical Science, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. Electronic address: f.giampieri@univpm.it.
9
Department of Odontostomatologic and Specialized Clinical Science, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. Electronic address: m.gasparrini@univpm.it.
10
Department of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: lada.zivkovic@pharmacy.bg.ac.rs.

Abstract

Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P < 0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P < 0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.

KEYWORDS:

17β-estradiol; Antigenotoxicity; Comet assay; DES; DOLE

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