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Curr Mol Med. 2019 Sep 27. doi: 10.2174/1566524019666190927155702. [Epub ahead of print]

New Insight Into Apolipoprotein A5 and the Modulation of Human Adipose-Derived Mesenchymal Stem Cells Adipogenesis.

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1
Department of Cardiovascular Medicine, the Second Xiangya Hospital of Central South University, Changsha, Hunan. China.

Abstract

BACKGROUND:

The hallmark of obesity is excessive accumulation of triglyceride (TG) in adipose tissue. Apolipoprotein A5 (ApoA5) has been shown to influence the prevalence and pathology of obesity. However, the underlying mechanisms remains to be unclarified.

METHODS:

Human adipose-derived mesenchymal stem cells (AMSCs) were treated with 600 ng/ml ApoA5. The effect of ApoA5 on affecting intracellular TG content and adipogenic related factors expression were determined. Furthermore, the effect of ApoA5 on CIDE-C expression was also determined.

RESULTS:

During the AMSCs adipogenesis, ApoA5 could reduce the intracellular lipid droplets accumulation and the TG level; meanwhile, ApoA5 down-regulated the expression level of adipogenic related factors, including CCAAT enhancer binding proteins α/β (C/EBPα/β), fatty acid synthetase (FAS) and fatty acid-binding protein 4 (FABP4). Furthermore, the suppression of adipogenesis by ApoA5 was mediated through the inhibition of CIDE-C expression, an important factor which could promote the process of human AMSCs adipogenesis. However, over-expressing intracellular expression of CIDE-C could lead to the loss-of-function of ApoA5 in inhibiting AMSCs adipogenesis.

CONCLUSIONS:

In conclusion, ApoA5 could inhibit human AMSCs adipogenesis through, at least partly, down-regulating CIDE-C expression. The present study provides insight into the mechanisms underlying the anti-obesity effect of ApoA5 and suggests that ApoA5 has the potential to prevent obesity by acting on AMSCs.

KEYWORDS:

Apolipoprotein A5; CIDE-C; adipogenesis; adipose-derived mesenchymal stem cells; inhibit

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