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Plant Physiol. 2019 Dec;181(4):1449-1458. doi: 10.1104/pp.19.00912. Epub 2019 Sep 25.

Identification of the Arabidopsis Calmodulin-Dependent NAD+ Kinase That Sustains the Elicitor-Induced Oxidative Burst.

Author information

1
Université Grenoble Alpes, Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Énergie Atomique et aux Énergies Alternatives (CEA), Institut National de la Recherche Agromique (INRA), Interdisciplinary Research Institute of Grenoble - Cell & Plant Physiology Laboratory (IRIG-LPCV), 38000 Grenoble, France.
2
Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland.
3
Université Grenoble Alpes, CEA, Institut National de la Santé et de la Recherche Médicale (INSERM), Interdisciplinary Research Institute of Grenoble - Exploring the DYnamics of Proteomes (IRIG-EDyP), 38000 Grenoble, France.
4
Department of Biosciences, University of Milan, 20133 Milan, Italy.
5
Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie (UMR1332 BFP), INRA, Université Bordeaux, 33882 Villenave d'Ornon, France.
6
MetaboHUB, Bordeaux, 33882 Villenave d'Ornon, France.
7
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, Université Paul Sabatier (UPS), BP 42617, 31326 Castanet-Tolosan, France.
8
Université Grenoble Alpes, Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Énergie Atomique et aux Énergies Alternatives (CEA), Institut National de la Recherche Agromique (INRA), Interdisciplinary Research Institute of Grenoble - Cell & Plant Physiology Laboratory (IRIG-LPCV), 38000 Grenoble, France gilles.curien@cea.fr.

Abstract

NADP(H) is an essential cofactor of multiple metabolic processes in all living organisms, and in plants, NADP(H) is required as the substrate of Ca2+-dependent NADPH oxidases, which catalyze a reactive oxygen species burst in response to various stimuli. While NADP+ production in plants has long been known to involve a calmodulin (CaM)/Ca2+-dependent NAD+ kinase, the nature of the enzyme catalyzing this activity has remained enigmatic, as has its role in plant physiology. Here, we used proteomic, biochemical, molecular, and in vivo analyses to identify an Arabidopsis (Arabidopsis thaliana) protein that catalyzes NADP+ production exclusively in the presence of CaM/Ca2+ This enzyme, which we named NAD kinase-CaM dependent (NADKc), has a CaM-binding peptide located in its N-terminal region and displays peculiar biochemical properties as well as different domain organization compared with known plant NAD+ kinases. In response to a pathogen elicitor, the activity of NADKc, which is associated with the mitochondrial periphery, contributes to an increase in the cellular NADP+ concentration and to the amplification of the elicitor-induced oxidative burst. Based on a phylogenetic analysis and enzymatic assays, we propose that the CaM/Ca2+-dependent NAD+ kinase activity found in photosynthetic organisms is carried out by NADKc-related proteins. Thus, NADKc represents the missing link between Ca2+ signaling, metabolism, and the oxidative burst.

PMID:
31554701
PMCID:
PMC6878019
[Available on 2020-12-01]
DOI:
10.1104/pp.19.00912

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