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Endosc Ultrasound. 2019 Sep 25. doi: 10.4103/eus.eus_36_19. [Epub ahead of print]

DNA sequencing of cytopathologically inconclusive EUS-FNA from solid pancreatic lesions suspicious for malignancy confirms EUS diagnosis.

Author information

1
Division of Endoscopy, Gastro Unit, Copenhagen University Hospital, Herlev, Denmark.
2
Department of Pathology, Copenhagen University Hospital Herlev, Herlev, Denmark.
3
Division of Surgery, Gastro Unit, Copenhagen University Hospital, Hvidovre, Denmark.

Abstract

Background and Objectives:

EUS-FNA is inconclusive in up to 10%-15% of patients with solid pancreatic lesions (SPLs). We aimed to investigate whether supplementary genetic analyses with whole-exome sequencing add diagnostic value in patients with SPLs suspicious of malignancy but inconclusive EUS-FNA.

Patients and Methods:

Thirty-nine patients, who underwent EUS-FNA of an SPL were retrospectively included. Three groups were defined: 16 (41.0%) had suspected malignancy on EUS confirmed by cytology (malignant), 13 (33.3%) had suspected malignancy on EUS but benign cytology (inconclusive), and 10 (25.6%) had benign EUS imaging and cytology (benign). Areas with the highest epithelial cell concentrations were macro-dissected from the FNA smears from each patient, and extracted DNA was used for whole-exome sequencing by next-generation sequencing of a selected gene panel including 19 genes commonly mutated in cancer.

Results:

Pathogenic mutations in K-RAS, TP53, and PIK3CA differed significantly between the three groups (P < 0.001, P = 0.018, and P = 0.026, respectively). Pathogenic mutations in KRAS and TP53 were predominant in the inconclusive (54% and 31%, respectively) and malignant groups (81.3% and 50%, respectively) compared to the benign group (0%). Malignant and inconclusive diagnoses correlated strongly with poor overall survival (P < 0.001).

Conclusion:

Whole-exome sequencing of genes commonly mutated in pancreatic cancer may be an important adjunct in patients with SPLs suspicious for malignancy on EUS but with uncertain cytological diagnosis.

KEYWORDS:

Diagnostics; EUS-FNA; next-generation sequencing; pancreatic ductal adenocarcinoma; whole-exome sequencing

PMID:
31552911
DOI:
10.4103/eus.eus_36_19
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