Pig Hsd17b3: Alternative splice variants expression, insertion/deletion (indel) in promoter region and their associations with male reproductive traits

J Steroid Biochem Mol Biol. 2019 Dec:195:105483. doi: 10.1016/j.jsbmb.2019.105483. Epub 2019 Sep 21.

Abstract

Hydroxysteroid 17-Beta Dehydrogenase 3 (Hsd17b3), primarily expressed in Leydig cells (LCs) of the mammalian testes, is essential for testosterone biosynthesis and male fertility. The aim of our study was to profile the expression, splice variants (SV) and novel insertion/deletion (indel) of Hsd17b3 in boars. Quantitative analysis showed that the expression level of Hsd17b3 in the testis was significantly highest. Among different testicular cell types, the Hsd17b3 mRNA expression level of LCs was significantly higher than that of SSCs (spermatogonial stem cells) and SCs (Sertoli cells). Furthermore, the SV was firstly identified in pigs and it was highly expressed in LCs comparing with SSCs and SCs. In addition, two mutations were identified in pig Hsd17b3 gene promotor and intron, respectively, which were associated with male reproductive traits (P < 0.05). In conclusion, both transcripts of Hsd17b3 gene were highly expressed in pig testes and LCs; the two novel indel variants of Hsd17b3 gene can be used as potential DNA makers for the marker-assisted selection in pigs. All these findings would enrich the study of Hsd17b3 gene in pig genetic breeding.

Keywords: Alternative splicing (AS); Genetic variants; Hydroxysteroid 17-Beta dehydrogenase (Hsd17b3); Leydig cells (LCs); Pig reproductive traits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 17-Hydroxysteroid Dehydrogenases / genetics*
  • Adult Germline Stem Cells / metabolism
  • Alternative Splicing
  • Animals
  • Genetic Variation
  • INDEL Mutation
  • Leydig Cells / metabolism*
  • Male
  • Promoter Regions, Genetic
  • Reproduction / genetics*
  • Sertoli Cells / metabolism
  • Sus scrofa / genetics*

Substances

  • 17-Hydroxysteroid Dehydrogenases