Format

Send to

Choose Destination
Dev Biol. 2019 Sep 21. pii: S0012-1606(19)30063-6. doi: 10.1016/j.ydbio.2019.09.008. [Epub ahead of print]

Ultra-low frequency labeling of single cell lineages in Drosophila.

Author information

1
Washington University School of Medicine, Department of Developmental Biology, 660 South Euclid Avenue St. Louis, MO, 63110, USA.
2
Washington University School of Medicine, Department of Developmental Biology, 660 South Euclid Avenue St. Louis, MO, 63110, USA. Electronic address: micchelli@wustl.edu.

Abstract

We describe a new methodology for genetically labeling single cell lineages in Drosophila called DMARCM. The system offers ultra-low frequency labeling, linear induction, consistent labeling among individuals and virtually no background signal. We compare this technique to an existing approach, which has been widely adopted. We demonstrate how application of DMARCM in the gastrointestinal epithelium permits the effects of labeling frequency on tumorigenic stem cell growth to be distinguished in an established tumor model.

KEYWORDS:

Cell lineage analysis; Drosophila; Monoclonality; Stem cells; Tumorigenesis

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center