Format

Send to

Choose Destination
Sci Rep. 2019 Sep 23;9(1):13718. doi: 10.1038/s41598-019-50078-3.

A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages.

Author information

1
Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand.
2
Fukujuji Hospital, Japan Anti-Tuberculosis Association (JATA), Kiyose, Japan.
3
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand.
4
Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi, Thailand.
5
Research Institute of Tuberculosis, JATA, Kiyose, Japan.
6
Genome Medical Science Project, National Center for Global Health and Medicine, Tokyo, Japan.
7
Chiangrai Prachanukroh Hospital, Ministry of Public Health, Chiangrai, Thailand.
8
TB-HIV Research Foundation, Chiangrai, Thailand.
9
Department of Biostatistics, Faculty of Public Health, Mahidol University, Bangkok, Thailand.
10
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
11
London School of Hygiene and Tropical Medicine, London, UK.
12
Welcome Trust Sanger Institute, Hinxton, Cambridge, UK.
13
Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand. Prasit.pal@mahidol.ac.th.
14
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand. Prasit.pal@mahidol.ac.th.

Abstract

Global Mycobacterium tuberculosis population comprises 7 major lineages. The Beijing strains, particularly the ones classified as Modern groups, have been found worldwide, frequently associated with drug resistance, younger ages, outbreaks and appear to be expanding. Here, we report analysis of whole genome sequences of 1170 M. tuberculosis isolates together with their patient profiles. Our samples belonged to Lineage 1-4 (L1-L4) with those of L1 and L2 being equally dominant. Phylogenetic analysis revealed several new or rare sublineages. Differential associations between sublineages of M. tuberculosis and patient profiles, including ages, ethnicity, HIV (human immunodeficiency virus) infection and drug resistance were demonstrated. The Ancestral Beijing strains and some sublineages of L4 were associated with ethnic minorities while L1 was more common in Thais. L2.2.1.Ancestral 4 surprisingly had a mutation that is typical of the Modern Beijing sublineages and was common in Akha and Lahu tribes who have migrated from Southern China in the last century. This may indicate that the evolutionary transition from the Ancestral to Modern Beijing sublineages might be gradual and occur in Southern China, where the presence of multiple ethnic groups might have allowed for the circulations of various co-evolving sublineages which ultimately lead to the emergence of the Modern Beijing strains.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center