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Int J Mol Sci. 2019 Sep 18;20(18). pii: E4625. doi: 10.3390/ijms20184625.

Cellular Mechanism Underlying rTMS Treatment for the Neural Plasticity of Nervous System in Drosophila Brain.

Luo Y1,2, Yang J3,4, Wang H5,6, Gan Z7,8, Ran D9,10.

Author information

1
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China. wolying@aliyun.com.
2
The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China. wolying@aliyun.com.
3
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China. cqyangjq@cqmu.edu.cn.
4
The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China. cqyangjq@cqmu.edu.cn.
5
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China. 101832@cqmu.edu.cn.
6
The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China. 101832@cqmu.edu.cn.
7
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China. gzj@cqmu.edu.cn.
8
Chongqing Research Center for Pharmaceutical Engineering, Chongqing Medical University, Chongqing 400016, China. gzj@cqmu.edu.cn.
9
Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China. 102832@cqmu.edu.cn.
10
The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China. 102832@cqmu.edu.cn.

Abstract

Repetitive transcranial magnetic stimulation (rTMS) is used as a research tool and clinical treatment for the non-clinical and clinical populations, to modulate brain plasticity. In the case of neurologic and psychiatric disease, there is significant evidence to suggest that rTMS plays an important role in the functional recovery after neurological dysfunction. However, the causal role for rTMS in the recovery of nervous dysfunction remains unclear. The purpose of the present study is to detect the regulation of rTMS on the excitatory neuronal transmission and specify the mode of action of rTMS on the neural plasticity using Drosophila whole brain. Therefore, we identified the effects of rTMS on the neural plasticity of central neural system (CNS) by detecting the electrophysiology properties of projection neurons (PNs) from adult Drosophila brain after rTMS. Using patch clamp recordings, we recorded the mini excitatory postsynaptic current (mEPSC) of PNs after rTMS at varying frequencies (1 Hz and 100 Hz) and intensities (1%, 10%, 50%, and 100%). Then, the chronic electrophysiology recordings, including mEPSC, spontaneous action potential (sAP), and calcium channel currents from PNs after rTMS at low frequency (1 Hz), with low intensity (1%) were detected and the properties of the recordings were analyzed. Finally, the frequency and decay time of mEPSC, the resting potential and frequency of sAP, and the current density and rise time of calcium channel currents were significantly changed by rTMS. Our work reveals that rTMS can be used as a tool to regulate the presynaptic function of neural circuit, by modulating the calcium channel in a frequency-, intensity- and time-dependent manner.

KEYWORDS:

calcium channel; excitatory neuronal transmission; projection neurons; repetitive transcranial magnetic stimulation; synaptic plasticity

PMID:
31540425
DOI:
10.3390/ijms20184625
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