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Stem Cell Res. 2019 Oct;40:101548. doi: 10.1016/j.scr.2019.101548. Epub 2019 Aug 23.

Generation of 3 clones of induced pluripotent stem cells (iPSCs) from a patient affected by Crohn's disease.

Author information

1
A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy. Electronic address: g.lanzi@unibs.it.
2
A. Nocivelli Institute for Molecular Medicine, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.
3
Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
4
Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34128 Trieste, Italy; Department of Medical, Surgical and Health Sciences, University of Trieste, 34149 Trieste, Italy.
5
Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34128 Trieste, Italy.

Abstract

Crohn's disease is a debilitating and incurable chronic inflammatory bowel disease, affecting millions of individuals worldwide, with an increasing frequency. Surgery must be applicable in half of the cases often with a disabling course, and pharmacological treatments may have adverse complications. We generated three isogenic clones of iPSCs from peripheral blood mononuclear cells (PBMCs) of a patient with Crohn's Disease under pharmacological treatment without adverse effects. Sendai virus based vector was used and the iPSCs were characterized for genetic uniqueness, genomic integrity, pluripotency, and differentiation ability. These iPSCs will be a powerful tool to develop tailored therapies.

PMID:
31536868
DOI:
10.1016/j.scr.2019.101548
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