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Nanoscale. 2019 Oct 7;11(37):17206-17210. doi: 10.1039/c9nr05234h. Epub 2019 Sep 17.

Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches.

Author information

1
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, China.
2
Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China. dczaj@sjtu.edu.cn.
3
School of Science, Ningbo University, Ningbo 315211, Zhejiang, China.
4
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
5
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China and School of Physical Science and Technology, Shanghai Tech University, Shanghai 201204, China.

Abstract

Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.

PMID:
31535117
DOI:
10.1039/c9nr05234h

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