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Rev Assoc Med Bras (1992). 2019 Sep 12;65(8):1067-1073. doi: 10.1590/1806-9282.65.8.1067.

TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes.

Author information

1
. PhD, Department of Anesthesia, Renmin Hospital, Wuhan University, China.
2
. Master, Department of Anesthesia, Renmin Hospital, Wuhan University, China.
3
. Department of Anesthesia, Renmin Hospital, Wuhan University, China.

Abstract

OBJECTIVE:

Diabetes is a risk factor for acute kidney injury (AKI). However, its mechanism of pathogenesis has not been elucidated. The aim of the study was to investigate the role of inflammation and the toll-like receptor 7 (TLR7) in ischemic AKI for diabetes.

METHODS:

A high glucose hypoxia-reoxygenation model of human renal tubular epithelial (HK-2) cells was used to generate AKI induced by ischemia-reperfusion in diabetes. The activity of cells was measured by CCK-8 assay and LDH activity. Inflammatory cytokines were assessed by ELISA. TLR7, MyD88, and NF-κB expressions were examined by western blotting. Apoptosis was evaluated by flow cytometry.

RESULTS:

The high glucose group and low glucose group were subjected to hypoxia-reoxygenation. The low glucose group developed only mild cell damage, apoptosis, and inflammatory response. In contrast, an equivalent hypoxia-reoxygenation injury provoked severe cell damage, apoptosis, and inflammatory response in the high glucose group. Expression of TLR7 and its related proteins were measured in the high glucose group before and after hypoxia-reoxygenation. The high glucose group exhibited more significant increases in TLR7 expression following hypoxia-reoxygenation than the low glucose group. In addition, the expression of TLR7 and its related proteins after hypoxia-reoxygenation were higher in the high glucose group than in the low glucose group. Inhibition of TLR7 provides significant protection against ischemic injury in diabetes.

CONCLUSION:

Our results suggest that diabetes increases the vulnerability to ischemia-induced renal injury. This increased vulnerability originates from a heightened inflammatory response involving the TLR7 signal transduction pathway.

PMID:
31531603
DOI:
10.1590/1806-9282.65.8.1067
[Indexed for MEDLINE]
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