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Planta. 2019 Sep 16. doi: 10.1007/s00425-019-03274-4. [Epub ahead of print]

Fruit metabolic and transcriptional programs differentiate among Andean tomato (Solanum lycopersicum L.) accessions.

Author information

1
Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR), Rosario, Argentina.
2
Animal Nutrition and Welfare Service, Animal and Food Science Department, Universitat Autónoma de Barcelona, 08193, Bellaterra, Spain.
3
Instituto de Fisiología Biología Molecular y Neurociencias (IFIBYNE-CONICET-UBA), Buenos Aires, Argentina.
4
Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina.
5
IADIZA CCT-CONICET, Mendoza, Argentina.
6
Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR), Rosario, Argentina. valle@ibr-conicet.gov.ar.

Abstract

Andean tomatoes differed from the wild ancestor in the metabolic composition and the expression of genes related with mitochondrial functions, and environmental stresses, making them potentially suitable for breeding programmes. Traditional landraces or "criollo" tomatoes (Solanum lycopersicum L.) from Andean areas of Argentina, selected for their fruit quality, were analysed in this study. We explored the metabolome and transcriptome of the ripe fruit in nine landrace accessions representing the seven genetic groups and compared them to the mature fruit of the wild progenitor Solanum pimpinellifolium. The content of branched- (isoleucine and valine) and aromatic (phenylalanine and tryptophan) amino acids, citrate and sugars were significantly different in the fruit of several "criollo" tomatoes compared to S. pimpinellifolium. The transcriptomic profile of the ripe fruit showed several genes significantly and highly regulated in all varieties compared to S. pimpinellifolium, like genes encoding histones and mitochondrial proteins. Additionally, network analysis including transcripts and metabolites identified major hubs with the largest number of connections such as constitutive photomorphogenic protein 1 (a RING finger-type ubiquitin E3 ligase), five Zn finger transcription factors, ascorbate peroxidase, acetolactate synthase, and sucrose non-fermenting 1 kinase. Co-expression analysis of these genes revealed a potential function in acquiring tomato fruit quality during domestication.

KEYWORDS:

Amino acids; Histones; Landraces; Mitochondria; Ripe fruit; Sugars

PMID:
31529400
DOI:
10.1007/s00425-019-03274-4

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