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Nat Methods. 2019 Sep 16. doi: 10.1038/s41592-019-0554-0. [Epub ahead of print]

The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue.

Author information

1
Brain Research Institute, University of Zurich, Zurich, Switzerland. voigt@hifo.uzh.ch.
2
Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland. voigt@hifo.uzh.ch.
3
University Hospital Zurich, Zurich, Switzerland.
4
Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.
5
Wyss Center for Bio- and Neuroengineering, Geneva, Switzerland.
6
Department of Basic Neurosciences, Geneva Neuroscience Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
7
Sainsbury Wellcome Centre for Neural Circuits and Behaviour, London, UK.
8
Brain Research Institute, University of Zurich, Zurich, Switzerland.
9
Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
10
Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
11
Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
12
ICM-Brain & Spine Institute, Paris, France.
13
Department of Brain and Behavioural Sciences, University of Pavia, Pavia, Italy.
14
Hungarian Academy of Sciences Research Centre for Natural Sciences, Budapest, Hungary.
15
Faculty of Information Technology and Bionics, Pazmany Peter Catholic University, Budapest, Hungary.
16
Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, Netherlands.
17
Friedrich Miescher Institute Basel, Basel, Switzerland.
18
Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
19
Department of Molecular Medicine, University of Pavia, Pavia, Italy.
20
Clinic of Neurology, Department of Clinical Neurosciences, Geneva University Hospital, Geneva, Switzerland.

Abstract

Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).

PMID:
31527839
DOI:
10.1038/s41592-019-0554-0

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