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Infect Immun. 2019 Sep 16. pii: IAI.00651-19. doi: 10.1128/IAI.00651-19. [Epub ahead of print]

The combinations-chitosan + Pam3CSK4 and chitosan + MPL: promising immune enhancing adjuvants for anti-caries vaccine PAc.

Bi Y1, Xu Q2,3, Su L4, Xu J5, Liu Z1, Yang Y1, Tang H6, Li Y7, Fan M8.

Author information

1
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
2
School of Medicine, Jianghan University, Wuhan, China.
3
Wuhan First Stomatological Hospital, Wuhan, China.
4
Department of Endodontics & Key Laboratory for Oral Biomedical Research of Zhejiang Province, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, China.
5
Taikang Tongji (Wuhan) Hospital, Wuhan, China.
6
Institute of Immunology, Taishan Medical University, Taian, China.
7
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China 1004809372@whu.edu.cn.
8
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China fmwmail@whu.edu.cn.

Abstract

We previously demonstrated that recombinant protein PAc could be administered as an anti-caries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan + Pam3CSK4 and chitosan + MPL in the immune responses to the PAc protein in vivo and in vitro. Compared with PAc alone, PAc + chitosan + Pam3CSK4 or PAc + chitosan + MPL promoted significantly higher PAc-specific antibody titers in serum and saliva, inhibited Streptococcus mutans (S. mutans) colonization onto the tooth surfaces and endowed better protection effect with significantly less caries activities. Chitosan + Pam3CSK4 and chitosan + MPL showed no statistic differences. In conclusion, our study demonstrated that the combination of chitosan + Pam3CSK4 or chitosan + MPL is promising for anti-caries vaccine development.

PMID:
31527122
DOI:
10.1128/IAI.00651-19

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