Format

Send to

Choose Destination
Toxicol In Vitro. 2019 Dec;61:104652. doi: 10.1016/j.tiv.2019.104652. Epub 2019 Sep 14.

Comparison of cytotoxicity of IQOS aerosols to smoke from Marlboro Red and 3R4F reference cigarettes.

Author information

1
Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, United States. Electronic address: barbara.davis@email.ucr.edu.
2
Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, United States. Electronic address: vto002@ucr.edu.
3
Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, United States. Electronic address: talbot@ucr.edu.

Abstract

This study compared the cytotoxicity of IQOS aerosols to smoke from Marlboro Red (MR) and 3R4F reference cigarettes. Aerosol/smoke solutions were tested as the gas vapor phase (GVP), particulate phase (total particulate matter or TPM), or whole aerosol/smoke (WA), the latter being what smokers actually inhale. Cytotoxicities were evaluated using the LDH, MTT and neutral red uptake (NRU) assays in conjunction with eight different cell types, mainly from the respiratory system. Most test solutions did not compromise the plasma membranes of cells (LDH). However, mitochondrial activity (MTT) and dye uptake/lysosomal activity (NRU) were equally depressed by IOQS aerosols and cigarette smoke solutions at the high concentrations. Our NRU data with mouse NIH/3T3 transformed fibroblasts were similar to those previously reported by the IQOS manufacturer and showed little cytotoxicity in the NRU assay. In both studies with NIH/3T3 cells, the results were significantly different from 3RF4 cigarette smoke, suggesting reduced toxicity with IQOS. However, by expanding evaluations to a broader spectrum of cells that included respiratory system cells and by including higher concentrations of GVP, as well as WA, cytotoxicity equivalent to that of Marlboro Red and 3R4F cigarettes was frequently observed with IQOS aerosols in the MTT and NRU assays.

KEYWORDS:

Cytotoxicity; Electronic nicotine delivery devices; Heat-not-burn tobacco products; Heated tobacco product; IQOS; New tobacco products

PMID:
31526836
DOI:
10.1016/j.tiv.2019.104652
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center