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J Oral Maxillofac Pathol. 2019 May-Aug;23(2):198-202. doi: 10.4103/jomfp.JOMFP_28_19.

Characterization of oral fibroblasts: An in vitro model for oral fibrosis.

Author information

1
Department of Oral Pathology, College of Dentistry, Gulf Medical University, Ajman, United Arab Emirates.
2
Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
3
Department of Oral and Maxillofacial Pathology, Ragas Dental College, Chennai, Tamil Nadu, India.
4
Research Center for Regenerative Medicine and Stem Cell Research, Central Research Facility, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
5
Department of Periodontics, College of Dentistry, Gulf Medical University, Ajman, United Arab Emirates.

Abstract

Background:

Oral submucous fibrosis (OSMF) is a chronic debilitating condition of the oral mucosa that has been classified as a potentially malignant disorder with a malignant transformation rate of 2%-8%. Several in vitro and in vivo experiments have been performed to formulate a treatment modality for OSMF, yet no ideal in vitro primary oral fibroblast model has been developed.

Aim:

To establish an in vitro primary oral fibroblast model.

Setting and Design:

In vitro laboratory setting.

Materials and Methodology:

Primary cell culture protocol was performed after obtaining normal oral tissue. Karyotyping was performed to rule out chromosomal abnormalities. Immunofluorescence staining was carried with a panel of fibroblast-specific markers (vimentin, phalloidin, transforming growth factor-β receptor 1 [TGFβR1] and s100a4) and Masson trichrome staining (MTS) to demonstrate the presence of extracellular matrix (ECM) qualitatively.

Results:

A monolayer of oral fibroblasts was observed on the 9th-day postseeding. No chromosomal abnormality was observed in the patient samples. Positive staining was observed with vimentin, phalloidin, TGFβR1 and s100a4, thereby confirming the cell type. MTS revealed fibroblasts with spindle morphology and scanty ECM.

Conclusion:

The present study lays down a protocol to design and characterize primary buccal fibroblast cell culture model that would aid researchers in performing in vitro preliminary experiments in areas concerning fibrosis.

KEYWORDS:

Fibrosis; oral fibroblasts; phalloidin; transforming growth factor

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