A persistent luminescence-based label-free probe for the ultrasensitive detection of hemoglobin in human serum

Yihui Liu; Yuhui Wang; Kai Jiang; Shan Sun; Sihua Qian; Qiaoping Wu; Hengwei Lin

doi:10.1016/j.talanta.2019.120206

A persistent luminescence-based label-free probe for the ultrasensitive detection of hemoglobin in human serum

Talanta. 2020 Jan 1:206:120206. doi: 10.1016/j.talanta.2019.120206. Epub 2019 Jul 31.

Authors

Yihui Liu¹, Yuhui Wang², Kai Jiang³, Shan Sun³, Sihua Qian³, Qiaoping Wu⁴, Hengwei Lin⁵

Affiliations

¹ School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China; Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.
² Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China. Electronic address: wangyuhui@nimte.ac.cn.
³ Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.
⁴ Ningbo Medical Center Lihuili Eastern Hospital, Ningbo, 315040, PR China. Electronic address: lhlyywqp@163.com.
⁵ Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China. Electronic address: linhengwei@nimte.ac.cn.

PMID: 31514889
DOI: 10.1016/j.talanta.2019.120206

Abstract

Hemoglobin (Hb) plays an important role in oxygen carriage for mammals, which is also a typical biomarker for certain diseases. Although numerous methods had been developed for the detection of Hb in red blood cells, analytical technology for the monitoring of low-abundance Hb in serum or plasma is still a challenge. Herein, persistent luminescence nanoparticles (PLNPs) with strong near-infrared (NIR) emission character behaving as a label-free probe for the highly sensitive and selective detection of Hb were developed. Further studies revealed that the sensing mechanism should be attributed to the Hb-induced dynamic quenching process. Moreover, the nanoprobe showed high selectivity to Hb against the common existing substances in human serum and a linear response to Hb ranging from 1 to 50 nM with an extremely high limit of detection (LOD) of 0.13 nM. Finally, applicability of the proposed probe for the detection of Hb in human serum samples was validated.

Keywords: Hemoglobin detection; Human serum sample; Label-free probe; Near-infrared emission; Persistent luminescence.

MeSH terms

Chromium / chemistry
Gallium / chemistry
Hemoglobins / analysis*
Humans
Limit of Detection
Luminescence
Luminescent Agents / chemistry*
Luminescent Measurements / methods
Metal Nanoparticles / chemistry*
Particle Size
Zinc / chemistry

Substances

Hemoglobins
Luminescent Agents
Chromium
Gallium
Zinc