Format

Send to

Choose Destination
J Appl Physiol (1985). 2019 Nov 1;127(5):1403-1418. doi: 10.1152/japplphysiol.00127.2019. Epub 2019 Sep 12.

Cold water immersion attenuates anabolic signaling and skeletal muscle fiber hypertrophy, but not strength gain, following whole-body resistance training.

Author information

1
School of Exercise and Nutrition Sciences, Deakin University, Melbourne, Australia.
2
Centre for Sport Research (CSR), Deakin University, Melbourne, Australia.
3
Department of Physiology, Australian Institute of Sport (AIS), Canberra, Australia.
4
Institute for Health and Sport (iHeS), Victoria University, Melbourne, Australia.
5
Department of Exercise and Sport Science, University of North Carolina, Chapel Hill, North Carolina.
6
Faculty of Health, Sport and Human Performance, University of Waikato, Hamilton, New Zealand.
7
School of Behavioural and Health Sciences, Australian Catholic University, Melbourne, Australia.
8
School of Exercise and Nutrition Sciences, Queensland University of Technology, Brisbane, Australia.
9
School of Medical and Health Sciences, Edith Cowan University, Joondalup, Australia.

Abstract

We determined the effects of cold water immersion (CWI) on long-term adaptations and post-exercise molecular responses in skeletal muscle before and after resistance training. Sixteen men (22.9 ± 4.6 y; 85.1 ± 17.9 kg; mean ± SD) performed resistance training (3 day/wk) for 7 wk, with each session followed by either CWI [15 min at 10°C, CWI (COLD) group, n = 8] or passive recovery (15 min at 23°C, control group, n = 8). Exercise performance [one-repetition maximum (1-RM) leg press and bench press, countermovement jump, squat jump, and ballistic push-up], body composition (dual X-ray absorptiometry), and post-exercise (i.e., +1 and +48 h) molecular responses were assessed before and after training. Improvements in 1-RM leg press were similar between groups [130 ± 69 kg, pooled effect size (ES): 1.53 ± 90% confidence interval (CI) 0.49], whereas increases in type II muscle fiber cross-sectional area were attenuated with CWI (-1,959 ± 1,675 µM2 ; ES: -1.37 ± 0.99). Post-exercise mechanistic target of rapamycin complex 1 signaling (rps6 phosphorylation) was blunted for COLD at post-training (POST) +1 h (-0.4-fold, ES: -0.69 ± 0.86) and POST +48 h (-0.2-fold, ES: -1.33 ± 0.82), whereas basal protein degradation markers (FOX-O1 protein content) were increased (1.3-fold, ES: 2.17 ± 2.22). Training-induced increases in heat shock protein (HSP) 27 protein content were attenuated for COLD (-0.8-fold, ES: -0.94 ± 0.82), which also reduced total HSP72 protein content (-0.7-fold, ES: -0.79 ± 0.57). CWI blunted resistance training-induced muscle fiber hypertrophy, but not maximal strength, potentially via reduced skeletal muscle protein anabolism and increased catabolism. Post-exercise CWI should therefore be avoided if muscle hypertrophy is desired.NEW & NOTEWORTHY This study adds to existing evidence that post-exercise cold water immersion attenuates muscle fiber growth with resistance training, which is potentially mediated by attenuated post-exercise increases in markers of skeletal muscle anabolism coupled with increased catabolism and suggests that blunted muscle fiber growth with cold water immersion does not necessarily translate to impaired strength development.

KEYWORDS:

adaptation; anabolism; catabolism; cold water immersion; resistance training

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center