Format

Send to

Choose Destination
Am J Clin Exp Urol. 2019 Aug 15;7(4):281-296. eCollection 2019.

Mass cytometry reveals species-specific differences and a new level of complexity for immune cells in the prostate.

Author information

1
Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.
2
Molecular Biology Interdepartmental Program, University of California Los Angeles, Los Angeles, CA, USA.
3
Division of Hematology-Oncology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
4
Department of Urology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
5
Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA, USA.
6
Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA, USA.
7
Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, USA.

Abstract

Chronic inflammation in the benign prostate has been associated with a higher risk of developing prostate cancer. While a range of immune lineages is found in the prostate including T cells, B cells and myeloid cells, the specific subsets of immune cells with each major lineage have not been well described. In this study, we use mass cytometry (CyTOF) to comprehensively and reproducibly profile immune cells in mouse and human prostate. Using 4 myeloid markers (CD11b, CD11c, F4/80, Ly6C) in the mouse, we identified 8 phenotypically-distinct myeloid populations, demonstrating considerable heterogeneity within the immune compartment of the mouse prostate. We then profiled the prostate immune microenvironment from 9 human patients. Unlike the mouse prostate which is myeloid-dominant, the immune compartment in the benign human prostate is consistently T-lymphocyte-dominant. Using the X-shift algorithm to identify individual immune subsets based on marker expression, we found 57 phenotypically-distinct immune cell types in the human prostate. Despite similar proportions of T, B and myeloid lineage cells in the benign human prostate of all patients evaluated, we observed considerable interpatient heterogeneity in the abundance of more specific immune subsets. These findings highlight the importance of studying the immune compartment in the prostate at a granular level and will lead to future studies addressing the functional role of specific immune subsets in prostate epithelial transformation.

KEYWORDS:

Prostate; immune; inflammation; lymphocyte; mass cytometry; myeloid

PMID:
31511834
PMCID:
PMC6734036

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center