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J Appl Microbiol. 2019 Sep 11. doi: 10.1111/jam.14443. [Epub ahead of print]

Antibiofilm efficacy of the gold compound auranofin on dual-species biofilms of Staphylococcus aureus and Candida spp.

Author information

1
Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, 410013, R.P. China.
2
Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, R.P. China.

Abstract

AIMS:

Staphylococcus aureus (a bacterial pathogen) and Candida spp. (opportunistic fungi) are two clinically relevant biofilm-forming microbes responsible for a majority of community- and nosocomial-acquired infections. Dual species biofilm formation between S. aureus and Candida spp. extremely enhances the antimicrobial resistance of the microorganisms and is difficult to treat with antibiotic therapy. Hence, it is crucial to explore new antimicrobial agents. Auranofin is a mixed ligand gold compound and has recently been repurposed as an antibacterial and antifungal agent. However, the effects of auranofin against dual species biofilm have remained largely untested.

METHODS AND RESULTS:

In the present study, by constructing biofilms on microplates and urinary catheter surfaces, auranofin showed strong planktonic cells and biofilm inhibitory effects against mono and dual culture models of S.aureus and Candida albicans but only exhibited moderate antibiofilm effects on Candida parapsilosis. Auranofin could be synergistic with subminimal inhibitory concentrations of amphotericinB against S. aureus + C. albicans/C. parapsilosis dual biofilms. Auranofin also showed effective antimicrobial effects on vancomycin-resistant strains. However, the antimicrobial effects of auranofin were decreased in the presence of heat-inactivated fetal bovine serum.

CONCLUSIONS:

In summary, auranofin could effectively inhibit S. aureus and C. albicans mono and dual biofilm formation in vitro.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Coexistence between Staphylococcus aureus and Candida spp. in dual biofilms leads to increased resistance to some conventionally used antimicrobials, indicating a need for alternative treatments. This study demonstrates the potential for the Au-containing compound auranofin in the treatment of dual biofilm infections and encourages further investigation of this treatment for clinical use.

KEYWORDS:

Candida albicans ; Candida parapsilosis ; Staphylococcus aureus ; auranofin; polymicrobial biofilm; urine catheter

PMID:
31509623
DOI:
10.1111/jam.14443

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