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Cell Biol Int. 2019 Sep 10. doi: 10.1002/cbin.11239. [Epub ahead of print]

Loss of Annexin A5 Expression Attenuates the Lipopolysaccharide-induced Inflammatory Response of Rat Alveolar Macrophages.

Author information

1
Department of Emergency, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
2
Department of Emergency, The PLA Army General Hospital, Beijing, China.

Abstract

Acute lung injury (ALI) is a common respiratory syndrome accompanied with inflammation response. Annexin A5 (AnxA5) has anti-thrombotic, anti-apoptotic, and anti-inflammatory properties. The current study aims to explore the potential effect of AnxA5 on lipopolysaccharide (LPS)-induced inflammatory response in alveolar macrophages (AMs). Rat alveolar macrophages (AMs) (NR8383) were used in this study, and the cell viabilities at 4, 8, and 16 h after LPS administration with gradient concentrations were determined using Cell Counting Kit-8 assay. Cell apoptosis and expressions of mRNAs and protein were determined by flow cytometry, quantitative real time (qRT)-PCR and western blot, respectively. We found that LPS suppressed the viability of AMs in a dose-dependent manner, and it elevated the expression of AnxA5 in AMs. Inhibition of AnxA5 improved the cell viability compared with LPS group and could reduce apoptosis rate in comparison with LPS treatment. The knockdown of AnxA5 suppressed the expressions of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 at both protein and mRNA levels and regulated the expressions of apoptosis-related molecules (Bax, Bcl-2 and cspases-3). Moreover, the knockdown of AnxA5 improved the expression levels of inhibitory kappa B (IκB) and nuclear factor E2-related factor 2 (Nrf2) but inhibited the expression of nuclear transcription factor kappa-B (NF-κB), compared with LPS group. SN50 and ML385 were used to validate this signaling, and the inhibition of AnxA5 suppressed LPS-induced inflammation, indicating that AnxA5 may be a potential anti-inflammatory target. Additionally, NF-κB/Nrf2 signaling pathway may also be involved in the LPS-induced inflammatory response of rat alveolar macrophages. This article is protected by copyright. All rights reserved.

KEYWORDS:

AnxA5 ; LPS; NF-κB; Nrf2; inflammation

PMID:
31502716
DOI:
10.1002/cbin.11239

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