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Mol Metab. 2019 Sep;27S:S1-S6. doi: 10.1016/j.molmet.2019.06.006.

Metabolically phenotyped pancreatectomized patients as living donors for the study of islets in health and diabetes.

Author information

1
Paul Langerhans Institute Dresden (PLID), Helmholtz Center Munich, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany; German Center for Diabetes Research (DZD e. V.), 85764 Neuherberg, Germany. Electronic address: marko.barovic@helmholtz-muenchen.de.
2
Department of Visceral-Thoracic-Vascular Surgery, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany. Electronic address: marius.distler@uniklinikum-dresden.de.
3
Paul Langerhans Institute Dresden (PLID), Helmholtz Center Munich, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany; German Center for Diabetes Research (DZD e. V.), 85764 Neuherberg, Germany. Electronic address: eyke.schoeniger@helmholtz-muenchen.de.
4
Paul Langerhans Institute Dresden (PLID), Helmholtz Center Munich, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany; German Center for Diabetes Research (DZD e. V.), 85764 Neuherberg, Germany; Department of Visceral-Thoracic-Vascular Surgery, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany. Electronic address: nicole.radisch@uniklinikum-dresden.de.
5
Department of Pathology, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany. Electronic address: daniela.aust@uniklinikum-dresden.de.
6
Department of Visceral-Thoracic-Vascular Surgery, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany. Electronic address: juergen.weitz@uniklinikum-dresden.de.
7
Vital-IT Group, SIB Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland. Electronic address: mark.ibberson@sib.swiss.
8
Sanofi-Aventis Deutschland GmbH, Diabetes Research, Industriepark Höchst, 65926 Frankfurt am Main, Germany. Electronic address: anke.schulte@sanofi.com.
9
Paul Langerhans Institute Dresden (PLID), Helmholtz Center Munich, University Hospital Carl Gustav Carus, Faculty of Medicine, TU Dresden, 01307 Dresden, Germany; German Center for Diabetes Research (DZD e. V.), 85764 Neuherberg, Germany; Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany. Electronic address: michele.solimena@tu-dresden.de.

Abstract

BACKGROUND:

The availability of human pancreatic islets with characteristics closely resembling those present in vivo is instrumental for ex vivo studies in diabetes research.

SCOPE OF REVIEW:

In this review we propose metabolically phenotyped surgical patients as a novel source of pancreatic tissue for islet research. Laser Capture Microdissection from snap frozen surgical specimens is a relatively simple, reproducible and scalable method to isolate islets of highest purity for many types of "omics" analyses. Fresh pancreatic tissue slices enable the functional characterization of living islet cells in situ through dynamic experiments. Access to complete medical history and laboratory values for each donor offers the opportunity of direct correlations with different "omics" data and detailed metabolic profiling prior to pancreas surgery. Peripheral blood samples complete the picture of each patient and represent a platform for pursuit of biomarkers with uniquely comprehensive background information in regard to the donor's islet cells.

MAJOR CONCLUSIONS:

Living donors provide the scientific community with a steady and abundant supply of excellent material to study islets closest to their in situ environment, thus advancing our understanding of their physiology in health and diseases.

KEYWORDS:

Biomarker; Diabetes; Islets of Langerhans; Laser capture microdissection; Metabolically phenotyped living donor; Pancreatectomy

PMID:
31500820
DOI:
10.1016/j.molmet.2019.06.006
Free PMC Article

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