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Chem Biol Interact. 2019 Sep 6:108819. doi: 10.1016/j.cbi.2019.108819. [Epub ahead of print]

Cannabidiol improves metabolic dysfunction in middle-aged diabetic rats submitted to a chronic cerebral hypoperfusion.

Author information

1
Biochemistry Department, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: mariarosazorzenon@hotmail.com.
2
Department of Pharmacology and Therapeutics, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: amandan.santiago@gmail.com.
3
Department of Pharmacology and Therapeutics, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: marcoaureliomori@gmail.com.
4
Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: silvanopionan23@gmail.com.
5
Biochemistry Department, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: clerjansen@gmail.com.
6
Biochemistry Department, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: eduardapp@hotmail.com.
7
Biochemistry Department, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: simone.ciotta@hotmail.com.
8
Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: pmathias@uem.br.
9
Centre for Interdisciplinary Research on Applied Neurosciences (NPNA), Department of Pharmacology, Medical School of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900 CEP, 14049-900, Ribeirão Preto, São Paulo, Brazil. Electronic address: fsguimar@fmrp.usp.br.
10
Department of Pharmacology and Therapeutics, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: rmmwoliveira@uem.br.
11
Biochemistry Department, State University of Maringa, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: pgmfernandes2@uem.br.
12
Department of Physiological Sciences, State University of Maringá, Av. Colombo 5790 CEP, 87020-900, Maringa, Parana, Brazil. Electronic address: cemcosta@uem.br.

Abstract

Cannabidiol (CBD), a compound obtained from Cannabis sativa, has wide range of therapeutic properties, including mitigation of diabetes and neurodegeneration. Cerebral ischemia and consequent learning disabilities are aggravated in elderly diabetic subjects. However, there are no studies showing the effect of CBD treatment in elderly diabetes patients suffering cerebral ischemia. The present work tested the hypothesis that CBD treatment improves metabolic dysfunctions in middle-aged diabetic rats submitted to chronic cerebral hypoperfusion. In this work, 350-day-old male Wistar streptozotocin-induced diabetic rats were used. To induce cerebral ischemia was used a chronic cerebral hypoperfusion (CCH), surgically, via the four-vessel occlusion/internal carotid artery (4-VO/ICA). Four diabetic groups were established: Non-CCH Treated Diabetic (DNT), CCH Treated Diabetic (DCT), Non-CCH Vehicle Diabetic (DNV), and CCH Vehicle Diabetic (DCV). Vehicle groups were not treated with CBD. The animals were treated during 30 days with 10 mg CBD/Kg bw/day. After treatment, the animals were euthanized, and blood levels of glucose, insulin, total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides, fructosamine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were evaluated. DCT group presented reduction of hyperglycemia and an increase of insulinemia. Also was observed lower fructosamine, LDL, HDL, triglycerides and total cholesterol levels. AST and ALT concentration were reduced in CBD treated groups. CBD may be used as therapeutic tool to protect metabolism against injuries from diabetes aggravated by cerebral ischemia.

KEYWORDS:

Cannabis sativa; Cerebral ischemia; Diabetes mellitus; Fructosamine; Insulin; Middle-aged rats

PMID:
31499052
DOI:
10.1016/j.cbi.2019.108819

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