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Cancer Manag Res. 2019 Aug 6;11:7405-7425. doi: 10.2147/CMAR.S210332. eCollection 2019.

Postoperative metastasis prediction based on portal vein circulating tumor cells detected by flow cytometry in periampullary or pancreatic cancer.

Author information

1
Department of General Surgery, Peking University Third Hospital, Beijing 100191, People's Republic of China.
2
Department of Hepatobiliary Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan 450003, People's Republic of China.
3
Medical and Health Analytical Center, Peking University Health Science Center, Beijing 100191, People's Republic of China.
4
Department of Clinical Research, Yikon Genomics Co. Ltd., Shanghai, People's Republic of China.

Abstract

Purpose:

The aim of this study was to evaluate the value of flow cytometry (FCM) detection of portal vein circulating tumor cells (CTCs) in predicting postoperative metastasis.

Methods:

Samples of portal venous blood and peripheral blood were collected from 39 patients during surgery, and CTCs were detected by FCM, with confirmation by laser confocal microscopy and single-cell sequencing.

Results:

Among all patients, a portal EpCAM+CD45- percentage ≥24.5×10-4 (P=0.06), peripheral EpCAM+CD45- count ≥97/5 mL (P=0.034), peripheral EpCAM+CD45- percentage ≥4.4×10-4 (P=0.042), and CA242≥3.5 U/mL (P=0.027) were significant predictors of metastasis. Further analysis showed that the portal EpCAM+CD45- ratio ≥24.5×10-4 is a predictor of metastasis (P=0.025) in pancreatic cancer after curative resection.

Conclusion:

CTCs detected by FCM in portal venous blood are of significant value for the prediction of postoperative metastasis in pancreatic or periampullary tumors.

KEYWORDS:

circulating tumor cells; flow cytometry; metastases; pancreatic cancer; portal vein

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