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Gene. 1988 Dec 20;73(2):305-18.

Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.

Author information

1
Division of Biological Sciences, University of Missouri, Columbia 65211.

Abstract

Foreign DNA fragments can be inserted into a minor coat protein gene of filamentous phage, creating a fusion protein that is incorporated into the virion; we call these particles "fusion phage". The foreign amino acids are displayed on the surface, allowing fusion phage bearing antigenic determinants from a target gene to be purified in infectious form by affinity to antibody directed against the gene product. Here we introduce fusion-phage vectors that accept foreign DNA inserts with little effect on phage function; and describe affinity purification of virions bearing a target determinant from a 10(8)-fold excess of phage not bearing the determinant, using minute amounts of antibody. These "antibody-selectable" vectors are a promising alternative to conventional expression systems for using antibodies to clone genes, though the ability to isolate rare clones from actual libraries remains to be demonstrated.

PMID:
3149606
DOI:
10.1016/0378-1119(88)90495-7
[Indexed for MEDLINE]

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