Format

Send to

Choose Destination
Methods Cell Biol. 2019;154:15-29. doi: 10.1016/bs.mcb.2019.05.009. Epub 2019 Jul 26.

Kidney harvesting and metabolite extraction for metabolomics studies in rodents.

Author information

1
Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, United States; Tri-Institutional PhD Program in Chemical Biology, New York, NY, United States.
2
Metabolomics Core, Mayo Clinic, Rochester, MN, United States.
3
Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, United States. Electronic address: irazabalmira.maria@mayo.edu.

Abstract

Elucidating the metabolic changes that accompany disease states via metabolomics analysis of tissues has become an important avenue of exploration in biomarker and therapeutic target discovery. Conventional harvesting techniques rely on post-euthanasia tissue harvest which introduces ischemic conditions and subsequent metabolome changes that may ultimately introduce artifacts into final analyses. In this chapter, we present protocols for low-ischemia time rapid kidney tissue harvest followed by metabolite extraction for metabolomics studies in rodents.

KEYWORDS:

Ischemia/reperfusion; Metabolomics; Tissue harvest

PMID:
31493816
DOI:
10.1016/bs.mcb.2019.05.009

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center