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Mech Ageing Dev. 2019 Oct;183:111146. doi: 10.1016/j.mad.2019.111146. Epub 2019 Sep 4.

Classical monocytes from older adults maintain capacity for metabolic compensation during glucose deprivation and lipopolysaccharide stimulation.

Author information

1
School of Health Studies, University of Memphis, 38152, USA.
2
School of Health Studies, University of Memphis, 38152, USA; Center for Nutraceutical and Dietary Supplement Research, University of Memphis, Memphis, TN 38152, USA. Electronic address: bdpence@memphis.edu.

Abstract

Inflammaging is the chronic low-grade inflammation that occurs with age that contributes to the pathology of age-related diseases. Monocytes are innate immune cells that become dysregulated with age and which can contribute to inflammaging. Metabolism plays a key role in determining immune cell functions, with anti-inflammatory cells primarily relying on fatty acid oxidation and pro-inflammatory cells primarily relying on glycolysis. It was recently shown that lipopolysaccharide (LPS)-stimulated monocytes can compensate for a lack of glucose by utilizing fatty acid oxidation. Given that mitochondrial function decreases with age, we hypothesized that classical monocytes taken from aged individuals would have an impaired ability to upregulate oxidative metabolism along with impaired effector functions. Aging did not impair LPS-induced oxygen consumption rate during glucose deprivation as measured on a Seahorse XFp system. Additionally, aged classical monocytes maintained inflammatory gene expression responses and phagocytic capacity during LPS stimulation in the absence of glucose. In conclusion, aged classical monocytes maintain effector and metabolic functions during glucose deprivation, at least in an ex vivo context.

KEYWORDS:

Classical monocytes; Immunometabolism; Inflammaging; Inflammation; Innate immunity

PMID:
31493436
DOI:
10.1016/j.mad.2019.111146
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