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Behav Brain Res. 2019 Dec 30;376:112210. doi: 10.1016/j.bbr.2019.112210. Epub 2019 Sep 4.

Proteomic and transcriptional profiling of rat amygdala following social play.

Author information

1
Center for Environmental Health Sciences, MS, USA; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS, USA.
2
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA.
3
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS, USA.
4
Department of Computer Engineering and Computer Science, KY, USA.
5
Department of Computer Engineering and Computer Science, KY, USA; KBRIN Bioinformatics Core, University of Louisville, KY, USA. Electronic address: juw.park@louisville.edu.
6
Center for Environmental Health Sciences, MS, USA; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS, USA. Electronic address: rlcarr@cvm.msstate.edu.

Abstract

Social play is the most characteristic form of social interaction which is necessary for adolescents to develop proper cognitive, emotional, and social competency. The information available on neural substrates and the mechanism involved in social play is limited. This study characterized social play by proteomic and transcriptional profiling studies. Social play was performed on male Sprague Dawley rats on postnatal day 38 and protein and gene expression in the amygdala was determined following behavioral testing. The proteomic analysis led to the identification of 170 differentially expressed proteins (p ≤ 0.05) with 67 upregulated and 103 downregulated proteins. The transcriptomic analysis led to the identification of 188 genes (FDR ≤ 0.05) with 55 upregulated and 133 downregulated genes. DAVID analysis of gene/protein expression data revealed that social play altered GABAergic signaling, glutamatergic signaling, and G-protein coupled receptor (GPCR) signaling. These data suggest that the synaptic levels of GABA and glutamate increased during play. Ingenuity Pathway Analysis (IPA) confirmed these alterations. IPA also revealed that differentially expressed genes/proteins in our data had significant over representation of neurotransmitter signaling systems, including the opioid, serotonin, and dopamine systems, suggesting that play alters the systems involved in the regulation of reward. In addition, corticotropin-releasing hormone signaling was altered indicating that an increased level of stress occurs during play. Overall, our data suggest that increased inhibitory GPCR signaling in these neurotransmitter pathways occurs following social play as a physiological response to regulate the induced level of reward and stress and to maintain the excitatory-inhibitory balance in the neurotransmitter systems.

KEYWORDS:

Amygdala; Behavior; Gene expression; Protein expression; Reward; Social play

PMID:
31493430
PMCID:
PMC6783381
[Available on 2020-12-30]
DOI:
10.1016/j.bbr.2019.112210

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