Efficient data acquisition with three-channel centerpieces in sedimentation velocity

Kristian Juul-Madsen; Huaying Zhao; Thomas Vorup-Jensen; Peter Schuck

doi:10.1016/j.ab.2019.113414

Efficient data acquisition with three-channel centerpieces in sedimentation velocity

Anal Biochem. 2019 Dec 1:586:113414. doi: 10.1016/j.ab.2019.113414. Epub 2019 Sep 4.

Authors

Kristian Juul-Madsen¹, Huaying Zhao², Thomas Vorup-Jensen³, Peter Schuck⁴

Affiliations

¹ Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA; Biophysical Immunology Laboratory, Department of Biomedicine, Aarhus University, Aarhus, Denmark.
² Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
³ Biophysical Immunology Laboratory, Department of Biomedicine, Aarhus University, Aarhus, Denmark.
⁴ Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. Electronic address: Peter.Schuck@nih.gov.

Abstract

Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record data from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.

Keywords: 3D printing; Analytical ultracentrifugation; Laboratory automation; Sedimentation velocity.

Published by Elsevier Inc.

Publication types

Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Automation
Printing, Three-Dimensional / instrumentation*
Software
Ultracentrifugation*

Grants and funding

ZIA EB000051-12/ImNIH/Intramural NIH HHS/United States