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Lasers Surg Med. 2019 Sep 7. doi: 10.1002/lsm.23155. [Epub ahead of print]

Photobiomodulation Increases Viability in Full-Thickness Grafts in Rats Submitted to Nicotine.

Author information

1
Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School-FMRP/USP.
2
Postgraduate Program in Pathology, Ribeirão Preto Medical School-FMRP/USP.
3
Postgraduate Program in Rehabilitation Sciences, Federal University of Santa Catarina-UFSC-Araranguá, Brazil.

Abstract

BACKGROUND AND OBJECTIVES:

Photobiomodulation (PBM) therapy with 830 nm wavelength or 660 wavelength to compare the effects with parameters of 30 mW, 0.028 cm2 , 9.34 seconds, and 3.64 J on the total integration of total skin grafts in rats submitted to nicotine.

STUDY DESIGN/MATERIALS AND METHODS:

Sixty male Wistar rats were divided in six groups: Sham-skin-grafting surgery; 830 nm-skin-grafting followed by 830 nm irradiation; 660 nm-skin grafting followed by 660 nm irradiation; Nicotine-subjected to subcutaneous nicotine injection (2 mg/kg twice a day for 4 weeks), followed by skin grafting; Group Nicotine/830 nm-similar to Group Nicotine, followed by 830 nm irradiation; Group Nicotine/660 nm-similar to Group Nicotine, followed by 660 nm irradiation. The percentage contraction of the grafting tissue was evaluated through ImageJ®. The thickness of the epidermis, inflammatory infiltrates, and the space between the implanted tissue and receptor bed were determined by histology; and the expression of vascular growth factor and blood vessel density (factor VIII) were evaluated by immunohistochemistry.

RESULTS:

The PBM at both wavelengths promoted a facilitating effect on the integration of the skin graft under nicotine and had a more significant effect on the thickness of the epidermis and expression of angiogenesis without nicotine at a wavelength of 830 nm. Different wavelengths influence responses related to the viability of cutaneous grafts in rats submitted to nicotine.

CONCLUSIONS:

The PBM with 830 nm and 660 nm promoted beneficial results in skin grafts submitted to the deleterious action of nicotine. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

KEYWORDS:

dosimetry; nicotine; photobiomodulation; skin; wound regeneration

PMID:
31493338
DOI:
10.1002/lsm.23155

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