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J Sci Food Agric. 2019 Sep 5. doi: 10.1002/jsfa.10020. [Epub ahead of print]

Standardized Emblica officinalis fruit extract inhibited the activities of alpha-amylase, alpha-glucosidase, dipeptidyl peptidase-4 and displayed antioxidant potential.

Author information

1
Sami Labs Limited, 19/1, 19/2, First Main, Second Phase, Peenya Industrial Area, Bangalore, 560 058, Karnataka, India.
2
Sabinsa Corporation, 20 Lake Drive, East Windsor, NJ, 08520, USA.
3
Sabinsa Corporation, 750 Innovation Circle, Payson, UT, 84651, USA.

Abstract

BACKGROUND:

Emblica officinalis known as amla in Ayurveda has been used as folk medicine to treat numerous pathological conditions including diabetes. However, the novel extract of E. officinalis fruit extract (Amla Fruit Extract, AFE, Saberry®) containing 100 gm kg-1 β-glucogallin along with hydrolyzable tannins has not yet been extensively studied for its anti-diabetic potential.

OBJECTIVE:

The aim of the current study was to investigate the anti-diabetic, anti-oxidant activities and stability during gastric stress as well as thermo-stability of AFE.

METHODS:

The effect of AFE on the inhibition of pancreatic α-amylase and salivary α-amylase enzymes was studied using starch and yeast α-glucosidase enzyme using 4-nitrophenyl α-D-glucopyranoside as substrate. Further, DPPH radical scavenging and ROS inhibition assay was performed against AFE.

RESULTS:

AFE potently inhibited the activities of α-amylase and α-glucosidase in a concentration dependent manner with the IC50 values of 135.70 and 106.70 μg/ml respectively. Furthermore, it also showed inhibition of α-glucosidase (IC50 562.9 μg/ml) and DPP-4 (IC50 3770 μg/ml) enzyme activities. AFE is a potent anti-oxidant showing a free radical scavenging activity with an IC50 , of 2.37μg/ml and protecting against cellular reactive oxygen species IC50 , of 1.77 μg/ml and the effects elicited could be attributed to its phytoconstituents.

CONCLUSION:

AFE showed significant gastric acid resistance and also found to be thermostable against the wet heat. Excellent α-amylase, α-glucosidase and DPP-4 inhibitory activities of AFE as well as antioxidant activities strongly recommend its use for the management of type 2 diabetes mellitus. This article is protected by copyright. All rights reserved.

KEYWORDS:

DPP-4; Saberry®; Type 2 diabetes mellitus; alpha amylase; alpha glucosidase; amla fruit extract; β-glucogallin

PMID:
31487036
DOI:
10.1002/jsfa.10020

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