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Methods Mol Biol. 2019;2054:223-241. doi: 10.1007/978-1-4939-9769-5_15.

Aptamer Selection for Detecting Molecular Target Using Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) Technology.

Author information

1
Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Shands Cancer Center, UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, FL, USA.
2
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, People's Republic of China.
3
Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, USA. jong.park@moffitt.org.

Abstract

Cell-SELEX is a live cell-based in vitro selection method that generates functional oligonucleotides, or aptamers. Often referenced as the chemist's antibody, aptamers bind to select targets with high affinity and can be utilized in a number of applications, including biomedicine, bioimaging, and biosensing. Here we describe the cell-SELEX technique and discuss this methodology's unique merit(s)-namely the ability to isolate highly selective aptamer panels with no prior knowledge of cellular signatures. This strategy thus presents as a technology that has the potential to enhance the precision of molecular medicine and targeted therapeutics.

KEYWORDS:

Aptamer; Cancer; Cell-SELEX; Chemical antibody; Functional oligonucleotide; Molecular science; Nucleic acid; Precision medicine; Targeted therapy; Theranostics

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